Uniklinik RWTH Aachen: GMP cryostorage for stem cells and cell therapy

Cryotechnology for reproductive medicine

In the stem cell laboratory at Uniklinik RWTH Aachen, preparations for cell therapy and GMP production are stored at approximately −180 °C in the gas phase above liquid nitrogen. Consarctic® planned, built, and commissioned the entire cryogenic infrastructure for this new facility in January 2026: a 16,000-liter external tank, 74.9 meters of vacuum-insulated piping, 13 sample storage containers, two freezers, and a control system that operates the entire nitrogen cycle without manual intervention.

In the video, Wolfgang Wetzstein, Head of Manufacturing at the stem cell laboratory of Uniklinik RWTH Aachen, explains how the laboratory operated previously and what has changed with the new facility.

Show transcript

I am the lab manager at IVF-SAAR. We are a private fertility clinic located in Saarbrücken. We perform about 900 egg retrievals and 450 cryo cycles. And we have been a customer of Consarctic for a very, very long time. That means I’ve been the lab manager here since 1997, almost 30 years. And I've been working together with Consarctic for just as long.

How the collaboration started, or especially how it deepened, was that we used to freeze pronuclear stages using the Planner. I know probably hardly anyone remembers that device anymore—a machine that was relatively loud and quite prone to breakdowns, so very sensitive. We were looking for a device with which we could freeze pronuclear stages, as well as sperm and testicular biopsies. The device that Consarctic offered was very small, made no noise at all, and was therefore ideal for the laboratory. You could use any type of carrier medium—straws, goblets, everything possible. For that reason, we went with Consarctic. We used that device for a very long time until vitrification came along, which basically replaced slow freezing and therefore gradually replaced that device as well. The device was very small, very quiet, and used very little nitrogen for freezing, and it could freeze all types of cells—whereas the Planner was very loud, consumed a lot of nitrogen, and was very disruptive.

Additionally, the more freezing you do—especially during the time when egg retrievals and cryopreservations were increasing—the more storage space we needed. With Consarctic, we immediately had a partner who could supply and serve us with cryo tanks. That made it a very nice symbiosis, so to speak. Because of that, we have worked together for a very long time up to this day. We’ve never had any problems, which I find very pleasant. With other companies, it’s sometimes problematic: you try to reach someone by phone, no chance; you send an email and nothing happens. That is really completely different with Consarctic. Mr. Been came by in person right at the beginning. You could tell right away: when the boss comes in person just for a single device sale, it shows that the managing director isn’t just interested in the money, but in the customer. Mr. Been is perceived as very, very personable. And it was the exact same with everyone else—we never had any issues. Everything happens very quickly. When I order something, I’ve rarely waited longer than two or three days.

The freezing rates were very good—meaning the thawing and survival rates—so the system was always very reliable, which allowed us to really help our patients because their cells survived. On top of that, we stored them in the tanks from Consarctic, where we never had any problems. Fortunately, and hopefully it stays that way. That provides a huge added value, because we were able to offer cryostorage and cryopreservation to give patients peace of mind. Naturally, the practice also benefits financially from that. Storage is something that is vital to patients so that their cells—their precious material—are preserved safely. We never had any worry that a problem would arise. That is, of course, a huge benefit for the practice, because that is the business of the practice. Anyone who works with a company for almost 30 years and wouldn't recommend them—that would be very surprising, I’d say. I would definitely recommend Consarctic. As I said, we’ve never had any issues with the cryo tanks, and they are simply reliable. We haven't had any problems with the equipment either. Therefore, I wouldn't know any reason why you shouldn't recommend them.

Aachen's cryobank by the numbers

13

Sample storage containers

74.9m

Installed equipment

16,000L

External tank

-180°C

Storage temperature

Why Uniklinik RWTH Aachen built a new facility

Until the end of 2025, the stem cell laboratory was supplied via individual feed tanks that fed into a nitrogen line. The sample storage containers were already refilling automatically, but only as long as liquid nitrogen was available from the feed tanks. To refill them, the dewars had to be transported across the university hospital.

This worked, but it regularly consumed staff time and tied the safety of the samples to a manual transport process. With seven containers, the system had also reached its capacity limit, and the technology was aging. The RWTH Aachen University Hospital issued a public tender for the cryogenic infrastructure for the new facility.

From capacity limit to automated cryostorage

Individual feed tanks, dewar transport across the hospital, and no room left for new samples: Consarctic has completely overhauled the university hospital's cryostorage. Today, a central external tank supplies all containers automatically, and there is space for 4,460 bags.
vs
Before consarctic:
7 sample storage containers
Supply via individual feed tanks
Dewar transport through the university hospital
Capacity limit reached
With consarctic
13 sample storage containers, 5,580 liters LN₂ capacity
16,000-liter external tank, filled by Linde
Fully automatic supply with no transport routes required
Designed for 4,460 bag storage positions

FAQ

Frequently asked questions about the cryogenic system in Aachen

What cryogenic technology does the stem cell laboratory at the University Hospital RWTH Aachen use?

The stem cell laboratory at the University Hospital RWTH Aachen operates with a Consarctic® system consisting of 11 BSR420+ cryo-containers, 2 BSF220+ containers, two BIOFREEZE® SMARTLINE and BV45 freezers, and a 16,000-liter external tank with 74.9 meters of vacuum piping. It is controlled via FMCS Touch and documented using Biolog®.

How long does it take to set up a cryogenic supply system?

In Aachen, two technicians took about three weeks for the installation, including the external tank, 74.9 meters of vacuum piping, exhaust piping, wiring, and commissioning.

The preceding planning and tendering phase is project-dependent and generally takes significantly longer than the installation itself.

What is gas-phase storage and why is it used for GMP specimens?

With gas-phase storage, samples are not submerged in liquid nitrogen but are kept in the cold gas above it—at approximately −180 °C in Aachen. Because no liquid medium connects the samples, cross-contamination between specimens is impossible—a prerequisite for storing clinical cell therapy products.

How can a cryogenic system be integrated into existing building management systems?

Via standardized interfaces: In Aachen, FMCS Touch reports all alarms to the university hospital's building management system and transmits fill levels and pressure values as a 4–20 mA signal.

Additionally, the control system receives alarms from the Dräger oxygen monitoring system and shuts off the nitrogen supply to the building in the event of oxygen deficiency.

Who builds turnkey cryogenic infrastructure for cell therapy facilities?

Consarctic GmbH plans, builds, qualifies, and maintains complete cryogenic systems for GMP facilities—from tender support to external tanks, piping, containers, and control systems, all the way to IQ/OQ documentation and maintenance contracts.

Consarctic® has been certified to EN ISO 13485 and ISO 9001 since 2004 and serves more than 1,500 customers in over 30 countries.

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Planning cryogenic infrastructure for your GMP laboratory

The project in Aachen demonstrates what a cryogenic supply system looks like when planned as a complete, integrated system from the start: storage, distribution, containment, freezing, monitoring, and occupational safety all work together seamlessly rather than being assembled from individual components.

The facility was commissioned in January 2026 and is now operational. The sample inventory from the legacy system will be transferred to the new containers in September 2026.

Are you planning a new build, an expansion, or the replacement of an existing facility? Consarctic® supports you from the initial site assessment and tender documentation through to IQ/OQ validation — complete with 24/7 emergency service.

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